Under the AMA style the first letter of the title and the subtitle must be capitalized. There has to be a single space and a colon between the title and subtitle. The rule of thumb is do not capitalize articles e.g. a, an, and the. Do not capitalize prepositions like as, at, off, to, as well as coordinating conjunctions, and, nor, but and for. Also do not capitalize the word to for infinitives

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